Journal: iScience

Article Title: Third-generation taxanes SB-T-121605 and SB-T-121606 are effective in pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2024.109044

Figure Lengend Snippet: In vitro efficacy of paclitaxel and SB-Ts (A) Graph showing the cell viability (% of control cells) of Paca-44 and BxPC-3 cell line treated with paclitaxel (PTX), second- and third-generation of SB-Ts for 72 h. Table with IC 50 values as mean ± S.D. of three independent experiments presented. (B) Representative graphs depicting the effect of concentrations corresponding to IC 50 (5–30 nM) 100 nM and 300 nM of PTX, SB-T-121605, and SB-T-12606 on cellular proliferation in Paca-44 and BxPC-3 cells. The data are presented as mean ± standard deviation of two independent experiments.

Article Snippet: Paca-44 (carrying KRAS G12V and TP53 C176S mutations) was obtained from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany (product. no. ACC 179) and the BxPC-3 cell line ( KRAS wild type) was obtained from CLS (Eppelheim, Germany, product no. 305031).

Techniques: In Vitro, Control, Standard Deviation

Journal: iScience

Article Title: Third-generation taxanes SB-T-121605 and SB-T-121606 are effective in pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2024.109044

Figure Lengend Snippet: Cell cycle changes after 24 h of incubation of cells with paclitaxel and experimental SB-Ts in vitro (A and B) Taxane concentrations corresponding to IC 50 values (5–30 nM) and 100 nM concentration were used in (A) Paca-44, (B) BxPC-3 cell line. Histograms display three independent experiments as mean ± SD.

Article Snippet: Paca-44 (carrying KRAS G12V and TP53 C176S mutations) was obtained from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany (product. no. ACC 179) and the BxPC-3 cell line ( KRAS wild type) was obtained from CLS (Eppelheim, Germany, product no. 305031).

Techniques: Incubation, In Vitro, Concentration Assay

Journal: iScience

Article Title: Third-generation taxanes SB-T-121605 and SB-T-121606 are effective in pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2024.109044

Figure Lengend Snippet: Western blot analysis of phospho-Bcl-2 (P-Bcl-2), PARP and cleaved caspase-3 expression in Paca-44 and BxPC-3 cells after paclitaxel and SB-Ts treatment (A) Cells treated with 25 nM (BxPC-3) or 30 nM (Paca-44) PTX, 5 nM SB-T-1216 (1216), 15 nM SB-T-121605 (121605), 5 nM SB-T-121606 (121606) or fresh medium without taxanes (CTRL) for 24 and 72 h. Expression of P-Bcl-2 (Ser70), PARP, and cleaved caspase-3 was analyzed employing western blot and relevant antibodies. Actin was used as a loading control. Data of one representative experiment out of three independent experiments shown. (B) Densitometric analysis of P-Bcl-2 (Ser70), cleaved form of PARP, and cleaved caspase-3. Data expressed as fold change of the respective control cells after 24 h and 72 h of treatment. The mean ± SD of three independent experiments shown. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Paca-44 (carrying KRAS G12V and TP53 C176S mutations) was obtained from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany (product. no. ACC 179) and the BxPC-3 cell line ( KRAS wild type) was obtained from CLS (Eppelheim, Germany, product no. 305031).

Techniques: Western Blot, Expressing, Control

Journal: iScience

Article Title: Third-generation taxanes SB-T-121605 and SB-T-121606 are effective in pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2024.109044

Figure Lengend Snippet: The analysis of cell death (A) Real-time monitoring by SYTOX Green with concentrations corresponding to IC 50 values (5–30 nM differing for each taxane-cell line combination), 100 and 300 nM PTX and SB-Ts. The data represent mean ± SD of three independent measurements. (B) The analysis of cell death by Annexin V and propidium iodide after 24, 48, and 72 h of incubation of cells with IC 50 and 100 nM concentrations of each taxane. All data presented as mean ± standard deviation. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Caspase 3 and 7 activity after 24 and 72 h of Paca-44 and BxPC-3 cells incubation with PXT and SB-Ts. Data are displayed as % of caspase activity in control cells incubated with cultivation medium only. All data presented as mean ± standard deviation. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Paca-44 (carrying KRAS G12V and TP53 C176S mutations) was obtained from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany (product. no. ACC 179) and the BxPC-3 cell line ( KRAS wild type) was obtained from CLS (Eppelheim, Germany, product no. 305031).

Techniques: Incubation, Standard Deviation, Activity Assay, Control

Journal: Cell Communication and Signaling : CCS

Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin

doi: 10.1186/s12964-019-0368-9

Figure Lengend Snippet: ER-472 antitumor activity and levels of active metabolite in 3 xenograft models; MIA PaCa-2, BxPC-3 and PC-3. Subcutaneous tumors were established in nude mice; treatment groups were n = 6 for MIA PaCa-2 and BxPC-3 and n = 5 for PC-3 efficacy studies and n = 3 for all metabolite studies. a , ER-472 was administered i.v. Q4Dx3 (dosing days denoted with long ticks on x-axis). Tumor volume data represents the mean ± SEM. b , mean active metabolite (S-methyl cryptophycin) tumor concentration versus time following a single dose of ER-472 at 2.5 mg/kg (MTD). Data represents the mean ± StdDev. c , antitumor activity of ER-472 versus ER-271 at molar equivalent (ME) dose in PC-3 model. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, one-way ANOVA followed by Tukey’s multiple comparison test; n.s. = not significant

Article Snippet: BxPC-3 cells (BW125058) were from Perkin Elmer (Waltham, MA), MIA PaCa-2 (ATCC Cat# CRM-CRL-1420, RRID:CVCL_0428) and PC-3 (ATCC Cat# CRL-7934, RRID:CVCL_0035) were from ATCC.

Techniques: Activity Assay, Concentration Assay

Journal: Cell Communication and Signaling : CCS

Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin

doi: 10.1186/s12964-019-0368-9

Figure Lengend Snippet: Correlation of ER-472 antitumor activity to NRP1 expression in tumor lysates in multiple xenograft models. Antitumor activity was assessed for ER-472 administered i.v. on a Q4Dx3 schedule, at ¼ to full MTD doses in multiple xenograft models. NRP1 expression in tumors was assessed by mRNA analysis and/or protein detection by western blot or IHC. Reasonable correlation was determined for 11 of the 13 models

Article Snippet: BxPC-3 cells (BW125058) were from Perkin Elmer (Waltham, MA), MIA PaCa-2 (ATCC Cat# CRM-CRL-1420, RRID:CVCL_0428) and PC-3 (ATCC Cat# CRL-7934, RRID:CVCL_0035) were from ATCC.

Techniques: Activity Assay, Expressing, Western Blot